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Facoltā di Scienze Agrarie e Alimentari Universitā degli Studi di Milano
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Genomics
Code: G6106-
Teacher:  Carlo Massimo Pozzi
Year: 
Term: 
CFU: 
CFU subdivision: Lectures: 6
Basic aims:  The course is meant to provide genomics and functional genomics tools, necessary to study and understand the molecular nature of plant (especially crops) genomes and gene functions. The student will be able to understand techniques in structural genomics and functional genomics. Moreover, the course will provide a methodological basis to apply those techniques in the frame of plant molecular breeding.
Acquired skills:  The student will acquire competences in structural genomics and functional genomics.
Course contents:  Program
The course will provide elements of genomics and functional
genomics. Genomics will be considered as a paradigmatic shift
in biological sciences, especially applied to plant genetics,
biology and breeding. The course will teach how to use
techniques and technologies to decypher genomes, understand
their structure and relationships between genomes of different
species. Elements of functional genomics will be provided. Also,
genomics will be studied in the context of breeding, and
bioinformatics tools will be presented that could help in
decoding and getting the most out of genomics info.
Program:  Program
Timing is provided as indicative and may vary according to
didactic necessities.
introduction (2 hours)
genomics as a paradigm shift (1 hours)
genome structure and how it is decoded (advanced sequencing
techniques)(5 hours)
genome decoding: reverse genetics (5 hours)
gene discovery (5 hours)
phenomics (2 hours)
RNAi and reverse genetics (3 hours)
Principles of functional genomics (6 hours)
Microarray as a specific tool in functional genomics (3 hours)
How genomics is used in breeding (8 hours)
Bioinformatics for genomics (6 hours)
Special tools and techniques (2 hours)
Prerequisites:  Please make sure that you are familiar with:
1. formal genetics (Mendel laws, for example)
2. recombination and creation of molecular maps
3. molecular markers
4. QTL and their mapping
5. basic methods for DNA sequencing
6. plant transgenesis
7. gene and genome editing basic techniques
8. principles of plant breeding (including elements of marker
assisted plant breeding)
Preparatory instructions:  Info not available
Learning materials:  Due to the innovative nature of the topic, a comprehensive and
updated text does not exist in Italian. You may want to get
acquainted with the topic using "Genetica e genomica (Barcaccia,
Falcinelli). Slides, reviews, papers and texts will be made available
on ARIEL.
Other info:  The exam can be written and oral, and will occur after the end
of the course. In general, if more than 4 students are
registered at any given exam session, the exam is going to be
written. Each written exam is composed of 6 questions.
Questions are open and usually not too specific (i.e. they allow
you to discuss in broad terms the answer). Each question can
score between 0-5. Oral exams lasts on average 30 minutes
and usually 6 questions are asked (again, questions are general
and open). The first question of an oral exam is quite often on
a subject of student's choice.
DOWNLOAD
Program of Genomics (pdf version)
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