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Facoltā di Scienze Agrarie e Alimentari Universitā degli Studi di Milano
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Virology and physiopathological biotechnologies-mod.2: physiopathological biotechnologies
Code: G590M-
Teacher:  Paola Casati
Year: 
Term: 
CFU: 
CFU subdivision: Lectures: 4
Practices in laboratory:  1
Other: 1
Basic aims:  The course is focused on plant viruses and virus-like diseases and on modern solving measures for their containment. Moreover, the course will offer knowledge on the application of biotechnology in the field of pest management in order to obtain protocols to be used for the containment of pathogens. The information and the achievements from the course will serve for the professional and management activities on the field of the plant protection.
Acquired skills:  The student will achieve information useful for the making decision and diagnostic processes. In addition the student will be able to propose appropriate measures for the control of diseases and to understand the implications of the use of biotechnology in plant protection.
Course contents:  Structure and physicochemical proprieties of virus
particles. Virus and host interactions, virus replication,
virus movement through the host plant. Host response to
virus infection. Virus transmission and control of plant
virus. Main Diseases of grapevine, fruits, cereal grains
and vegetable crops.
Program:  Introduction: economic losses due to plant viruses and
their distribution in the world. Definition of a Virus.
Structure and physicochemical proprieties of virus
particles. (proprieties of viral nucleic acid, viral protein).
Virus taxonomy: criteria used for classifying viruses.
Some important families and genera of plant viruses.
Viroids a Satellite RNAs: proprieties. Virus and host
interactions : Virus replication, virus movement through
the host plant. Host responses to virus infection: Effects
on plant metabolism and symptoms appear. Virus
transmission by insects, mites, nematodes, fungi.
Mechanical transmission. Transmission by vegetative
propagation, grafting, dodder, seed and pollen. Control of
plant virus: control or avoidance of vector, removal of
source of infection, propagation and maintenance of virus
free stocks, use of resistant plant (conventional
resistance transgenic plant- pathogen derived resistance).
Diseases: Fanleaf, Grapevine Leaf roll, Rugose wood,
Fleck. Plum Pox, Prunus Necrotic Ring Spot, Prunus
Dwarf, Cucumber Mosaic, Tomato Yellow Leaf Curl ,
Tomato Spottet Wilt, Potato Leafroll, Potato Spindle Tuber
(Viroid), Beet Necrotic Yellow Vein, Barley Yellow Dwarf,
Barley Yellow Mosaic, Wheat Streak Mosaic, Soil Borne
Wheat Mosaic, Maize Dwarf Mosaic Maize rough Dwarf,
Chrysantemum (Stunt). Practical aspects: symptom
recognition, biological assay (mechanical inoculation)
Prerequisites:  Knowledge of genetics and biochemistry and entomology
Preparatory instructions:  Agricultural botany,Biochemistry and Plant Physiology,
Agronomy, Genetics and breeding, Entomology
Learning materials:  MATTHEWS Plant Virology Academic Press 4th Edition.
GIUNCHEDI Malattie da virus, viroidi e fitoplasmi degli
alberi da frutto Edizioni Agricole de Il Sole 24 ore
Bologna. CONTI et al. I principali virus delle piante
ortive Bayer. GIUNCHEDI Elementi di Virologia
Vegetale Piccinin. BELLI Elementi di patologia
vegetale. Additional reading will be provide by the
Professor as scientific papers and web site
Other info:  The exam consists of an oral test with questions related
to the topics presented during the course
including laboratory and field classes
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Program of Virology and physiopathological biotechnologies-mod.2: physiopathological biotechnologies (pdf version)
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