Logo Facoltà di Scienze Agrarie e Alimentari Universitā degli Studi di Milano foto
Facoltā di Scienze Agrarie e Alimentari Universitā degli Studi di Milano
contatti    cerca    mappa   
Plant Pathology Diagnostic Laboratory - Module 2
Code: G-02BB
Teacher:  Fabio Quaglino
Year:   
Term: 
CFU: 
CFU subdivision: Lectures: 2
Practices in laboratory:  1
Basic aims:  The main objective of the course is to supply the information requested for performing the diagnosis of the plant diseases. Based on the topics described during the lessons and the laboratory trainings, the student will optimize his capability to select the work strategy suitable to formulate the diagnostic report.
Acquired skills:  Identification of main symptom patters of plant diseases caused by fungi, bacteria, phytoplasmas, viruses, and viroids; (ii) competence in challenging the diagnostic process steps: field surveys, sample collection and preparation for the laboratory analyses; (iii) Utilization of diagnostic tools employed for pathogen detection and identification (biological and traditional methods, serological methods, molecular analyses); (iv) employment of the criteria utilized for evaluating the diagnostic tools (efficiency, specificity, sensitivity); (v) interpretation of obtained data analyses and statement of the diagnostic report.
Course contents:  Diagnostic tools employed for pathogen detection and identification: (i) biological and traditional methods (optical/electronic microscopy, pathogen isolation, Koch postulates, transmission to test plants); (ii) serological methods (mono- and poly-clonal antibodies, ELISA); (iii) molecular analyses (hybridization; amplification: PCR, RT-PCR, IC-PCR, real-time PCR, LAMP; nucleotide sequence analyses: LH-PCR, RFLP, microsatellites, sequencing, in silico analyses). Criteria for evaluating the diagnostic tools: efficiency, specificity, sensitivity. Data analyses and diagnostic report statement.
LAB. TRAINING: pathogen identification by employing different diagnostic tools; evaluation of utilized tools based on studied criteria; diagnostic report statement.
Program:  Main diagnostic tools for detecting and identifying plant pathogens (viruses, phytoplasmas, bacteria, fungi, viroids) will be described: (i) biological and traditional methods (optical/electronic microscopy, pathogen isolation, Koch postulates, transmission to test plants); (ii) serological methods (mono- and poly-clonal antibodies, ELISA); (iii) molecular analyses (hybridization; amplification: PCR, RT-PCR, IC-PCR, real-time PCR, LAMP; nucleotide sequence analyses: LH-PCR, RFLP, microsatellites, sequencing, in silico analyses). Attention will focus on: (i) design of pathogen-specific diagnostic assays (biological and genetic features of the pathogen determine the choice of the appropriate method); (ii) utilization of positive and negative controls for evaluating the assay specificity and the presence of possible contamination; (iii) evaluating efficiency, specificity and sensitivity of the diagnostic tools; (iv) data analyses for establishing the diagnostic report. Furthermore, strategies for disease management, fixed by Government regulation, will be illustrated (this topic will be discussed more accurately in the module 1 of the course).
Notions explained during the lessons will be applied in laboratory training activities focused on evaluating different diagnostic methods used for identifying one pathogen selected as case study. In detail, the experiments will be carried out for detecting phytoplasmas. Obtained results will allow to (i) detect and identify the pathogen, and (ii) select the best diagnostic tool based on the efficiency, the specificity and the sensitivity of the assays employed.
Prerequisites:  It is recommended that the students have a knowledge of the plant pathology basics. In fact, it is requested the knowledge of the main features of the plant pathogens (not accurately explained during this course) for understanding the topics illustrated throughout the lessons.
Preparatory instructions:  No preparatory exams are mandatory, but it is recommended to attend this course after the exam in plant pathology.
Learning materials:  Powerpoint slides showed during the course and supplied scientific papers.
Other info:  The exam will be carried out as oral exam together with the professor of the course module 1. Therefore, the students have to know the topics described in both the modules during the lessons and the laboratory trainings.
DOWNLOAD
Program of Plant Pathology Diagnostic Laboratory - Module 2 (pdf version)
back
  The Faculty
  How to reach us
  Undergraduate programmes
  Courses
  Teaching organization
  LLP/Erasmus for incoming students
  Contacts
Universitā degli Studi di Milano