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Facoltā di Scienze Agrarie e Alimentari Universitā degli Studi di Milano
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Microbiological techniques
Code: G-012-
Teacher:  Maria Grazia Fortina
CFU subdivision: Lectures: 3
Practices in laboratory:  1
Basic aims:  Main purpose: to supply the cultural and operative instruments necessary for relating the acquired knowledge in the microbiological field to the microbiological methods and their application in the field of biotechnology.
Acquired skills:  Planning, organization and carriyng out of experimental analyses for the phenotypic and genotypic chracterization of microorganisms involved in the food industry.
Course contents:  Microbiological tests for the individuation and determination of microbial population in food matrix Planning and carriyng out of phenotypic and genotypic experiments for microbial identification Genetic studies for the exploitation of specific operation on bacterial genome of biotechnologically important microorganisms
Program:  • Lectures Isolation, selection and survey of microorganisms from heterogeneous matrices. Taxonomic identification of bacteria: phenotypic and genotypic characteristics. Planning and choise of experimental analyses for phenotypic and genotypic identification. Genetic organization in bacteria. The measurable characteristics of plasmidic and chromosomal DNA. The concepts of molecular genetics and their application in both basic and applied biotechnology (with particular regard to techniques that allow precise detection and amplification of DNA through the polymerase chain reaction or PCR) • Laboratory exercises Culture media preparation. Use of autoclaves and filters for the sterilization of media and materials. Isolation techniques. Measurement of bacterial growth. Obtainment of pure cultures. Planning and carrying out of phenotypic analyses for bacterial identification and characterization. Extraction of chromosomal and plasmidic DNA and their study: separation and visualization through gel electrophoresis techniques; % GC evaluation, determination of % of genetic homology
Prerequisites:  Info not available
Preparatory instructions:  General and applied Microbiology, Biochemistry
Learning materials:  Notes of the lectures and exercises Teaching material
Other info:  The oral examination consists in a discussion on the topics of the course, to verify the skills acquired
Program of Microbiological techniques (pdf version)
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